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. 2015 Oct 13;172(20):4790–4805. doi: 10.1111/bph.13250

Figure 2.

Figure 2

CBD was a NAM of arrestin2 recruitment to CB1 receptors following THC and 2‐AG treatment. HEK 293A (A–E) and STHdh Q7/Q7 (F–J) cells were transfected with arrestin2‐Rluc‐containing and CB1‐GFP2‐containing plasmids, and BRET2 was measured 30 min after treatment with 2‐AG or THC ± O‐2050 or CBD. CRCs were fit using Gaddum/Schild EC50 shift (A, B, F and G) and operational model of allosterism (C, D, H and I) nonlinear regression models. (E and J) Schild regressions were plotted as the logarithm of 2‐AG or THC dose against the logarithm of the dose–response at EC50 – 1. N = 6.