Table 3.

Effect of CBD on PLCβ3 activation in HEK 293A and STHdh ^Q7/Q7 cells

Agonist	CBD (μM)	EC50 μM (95% CI)a	E max (95% CI)a, b	n (95% CI)a, c	RA ± SEMd
HEK 293A
THC	DMSO	0.47 (0.27–0.69)	1.01 (0.82–1.20)	1.00 (0.76–1.26)	1.00 ± 0.0
	0.01	0.58 (0.34–0.81)	0.98 (0.80–1.17)	0.83 (0.70–1.13)	0.79 ± 0.17
	0.10	0.76 (0.59–0.97)	0.97 (0.81–1.13)	0.73 (0.67–0.93)	0.60 ± 0.08*
	0.50	0.86 (0.70–1.07)†	0.85 (0.70–1.00)	0.54 (0.41–0.72)†	0.46 ± 0.05*
	1.00	1.23 (0.85–1.80)†	0.71 (0.63–0.79)†	0.36 (0.18–0.51)†	0.27 ± 0.03*
	5.00	1.26 (0.82–1.58)†	0.51 (0.41–0.61)†	0.16 (0.04–0.26)†	0.19 ± 0.02*
2‐AG	DMSO	0.48 (0.28–0.72)	1.09 (0.90–1.29)	1.00 (0.86–1.15)	1.00 ± 0.0
	0.01	0.63 (0.37–0.96)	1.11 (0.91–1.30)	0.92 (0.81–1.02)	0.84 ± 0.07
	0.10	0.83 (0.58–1.03)	1.03 (0.74–1.32)	0.84 (0.74–1.00)	0.60 ± 0.07*
	0.50	1.11 (0.95–1.35)†	0.95 (0.80–1.10)	0.57 (0.46–0.79)†	0.41 ± 0.08*
	1.00	1.62 (1.23–1.51)†	0.78 (0.67–0.88)†	0.22 (0.07–0.36)†	0.23 ± 0.01*
	5.00	2.48 (1.72–3.22)†	0.60 (0.54–0.66)†	0.13 (0.04–0.24)†	0.12 ± 0.06*
STHdh Q7/Q7
THC	DMSO	0.58 (0.42–0.79)	0.77 (0.65–0.89)	1.00 (0.71–1.25)	1.00 ± 0.0
	0.01	0.72 (0.61–0.85)	0.73 (0.63–0.82)	0.54 (0.44–0.82)	0.77 ± 0.3
	0.10	0.99 (0.78–1.22)	0.62 (0.54–0.69)	0.51 (0.42–0.78)	0.48 ± 0.1*
	0.50	1.22 (0.85–1.57)†	0.53 (0.48–0.58)†	0.55 (0.23–0.64)†	0.33 ± 0.1*
	1.00	4.00 (2.76–4.32)†	0.49 (0.37–0.52)†	0.51 (0.17–0.62)†	0.10 ± 0.0*
	5.00	>5.00	–	<0.50	0.03 ± 0.0*
2‐AG	DMSO	0.66 (0.40–0.85)	0.73 (0.59–0.87)	1.00 (0.70–1.18)	1.00 ± 0.0
	0.01	0.67 (0.48–0.86)	0.65 (0.56–0.74)	0.77 (0.55–0.89)	0.88 ± 0.2
	0.10	0.78 (0.58–1.01)	0.61 (0.52–0.70)	0.57 (0.34–0.74)	0.71 ± 0.2*
	0.50	0.87 (0.63–0.92)	0.52 (0.46–0.58)†	0.39 (0.15–0.58)†	0.60 ± 0.1*
	1.00	1.04 (0.87–1.61)†	0.51 (0.43–0.56)†	0.39 (0.12–0.50)†	0.45 ± 0.1*
	5.00	1.78 (1.07–2.05)†	0.42 (0.32–0.51)†	0.36 (0.09–0.49)†	0.21 ± 0.0*

CI, confidence interval.

Data shown are means ± SEM or with 95% CI, from six independent experiments.

^aDetermined using nonlinear regression with variable slope (four parameters) analysis.

^bMaximal agonist effect BRET_Eff.

^cHill coefficient.

^dRelative activity, as determined in equation (2).

^† Significantly different from the DMSO vehicle as determined by non‐overlapping CI.

^* P < 0.01, compared with DMSO vehicle; one‐way ANOVA with Dunnett's multiple comparison test.