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. 2015 Oct 14;172(20):5009–5023. doi: 10.1111/bph.13270

Figure 6.

Figure 6

Immunofluorescence staining of NF‐κB in neurons, astrocytes and neutrophils in different groups at 24 h after cerebral ischaemia. When NF‐κB entered the nucleus, the combined figure would give rise to the purple colour. Scale bar = 100 µm. (A) Brain sections were triple‐stained with anti‐NF‐κB (red), anti‐Neun (green) and Hoechst 33342 (blue) to mark NF‐κB, neurons and nucleus. Arrows indicate the location of NF‐κB in neurons. (B) Brain sections were triple‐stained with anti‐NF‐κB (red), anti‐GFAP (green) and Hoechst (blue) to mark NF‐κB, astrocytes and nucleus. Arrows indicate the location of NF‐κB in astrocytes. (C) Brain sections were triple‐stained with anti‐NF‐κB (red), anti‐MPO (green) and Hoechst (blue) to mark NF‐κB, neutrophils and nucleus. Arrows indicate the location of NF‐κB in neutrophils.