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. 2015 Aug 7;10(9):861–871. doi: 10.1080/15592294.2015.1075691

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Figure 3. — H3 enrichment in HFD- and LFD-treated mice spermatozoa. (A) Distribution of H3 enrichment peaks among gene promoter regions, which include 5 kb upstream and 3 kb downstream the TSS, 5′UTR, 3′UTR, and coding sequence (CDS) in HFD7 vs. LFD8. (B) Heatmap of H3-ChIP signal intensities, normalized for input, around the TSS of protein-coding genes (23,350) in HFD7 and LFD8, at a 5 bp resolution. Profiles show the TSS plus 2 kb of upstream and 2 kb of downstream flanking region. Genes are ranked based on the number of CG sites. (C) Heatmap of H3-enrichment around the TSS of genes encoding microRNAs (1,516) in HFD7 and LFD8 samples. (D) Genome browser view presenting H3-enrichment, normalized to input, in HFD7 and LFD8 samples at genes (black) and CG islands (green bar).