Figure 1.

NVP-BEZ235 is cytotoxic only against osteosarcoma cells. Growth of MG-63 (A and B), U2OS (C and D) and SaOs-2 (E and F) osteosarcoma cells or MC3T3-E1 osteoblastic cells (I) with or without indicated NVP-BEZ235 (NVP) treatment was analyzed by MTT assay. MG-63 cells were treated with indicated concentration of NVP-BEZ235, trypan blue staining (G, 72 hrs) and “clonogenicity” (I, 10 days) were performed to test cell death. The data in this and all following figures were representatives of 3 different experiments. n=5 for each assay. The values were expressed as the means ±SD (Same for all figures). “C” stands for untreated control group (Same for all figures). *P < 0.05 vs. group “C.”