Figure 4.

Signaling changes by NVP-BEZ235 in osteosarcoma cells. MG-63 cells or U2OS cells were left untreated (“C”), or stimulated with NVP-BEZ235 (NVP, 200 nM) for 12 hrs, expression of listed proteins was tested by Western blots using corresponding antibodies (A-D, F-I). Phosphorylation of P85 and Akt was quantified and normalized to “C” (E), Phosphorylation of Erk and cyclin D1 expression was similar quantified (J). *P < 0.05 vs. group “C.”