Figure 2.

Interference of PTEN boosted CCL2 production and then promoted 4T1 tumor cells invasion and VEGF-A expression in vitro. (A) Production of CCL2 in TSN-exposed RAWs with shPTEN and pEGFP-PTEN was determined by ELISA. Supernatants were obtained for the indicated time periods, #P < 0.05 vs. control. (B) The 4T1 cells were added into the upper chamber and incubated for 24 h with different stimuli in culture medium. The migrated cells were quantified in 10 random fields at × 100 magnification. #P < 0.05. All data were representatives of at least 3 independent experiments. (C, D) mRNA and protein were extracted from TSN-exposed RAWs and U937 treated with siPTEN and LY294002, then validated the expression of PTEN, VEGF-A, and HIF-1α by Q-PCR and WB. Q-PCR and immunoblotting data were representatives of 3 separate experiments, #P < 0.05.