Skip to main content
. 2015 Feb 18;16(1):170–175. doi: 10.4161/15384047.2014.972765

Figure 2.

Figure 2.

VEGF-A and cMET pathways interact at the molecular level. (A) Representative image of western blot showing expression of VEGFR2, cMET and VEGF-A for vehicle only and for siRNAs targeting VEGFA and MET genes in primary human SCs. (B) Protein expression of VEGF-A, cMET and VEGFR2 after VEGFA and MET siRNA treatment of cultured human SCs (n = 2–4 different cultures) and VS cells (n = 5 different cultures) quantified through protein gel blot analysis. All levels are normalized to vehicle only protein expression, being 100% (dashed line). *P < 0 .05 **P < 0 .01. Error bars represent SEM. (C) Representative pictures of primary human VS cells treated with (a) vehicle only, (b) VEGFA siRNA, (c) MET siRNA, (d) DMSO only or (e) SU11274. BrdU in nuclei (red) marks proliferating cells, nuclei are labeled with DAPI. Scale bar = 100 μm for all images. (D) Quantification of proliferation changes after siRNA (n = 5 different cultures) and after SU11274 (n = 4 different cultures) treatment of primary VS cells normalized to proliferation in control non-treated cells. *P < 0 .05, **P < 0 .01. re = in comparison to. Error bars represent SEM.