Figure 2.

FcRn binding rate determined in vitro was compared to the human half-life previously determined in Phase 1 clinical studies. Using the BLI platform described in Materials and Methods, the fold change FcRn binding rate of 5 human IgG1 mAbs was independently determined and plotted against its known human half-life to establish a linear correlation. Values are the mean ± SEM. Line of best fit is shown in black with 95% confidence interval in gray. For in vitro experiments the average of at least 3 independent experiments is shown (N = 5 within each independent experiment). See Materials and Methods for details of clinical data.