Figure 1.

IgG1 antibodies mediate cytotoxicity and IFNγ cytokine secretion by PBMCs. (A) ADCC was performed using human PBMC as effector cells and MDA-MB-231 cells as targets with the indicated concentrations of anti-CD142 antibodies. Data are representative of 2 independent experiments (duplicate measurements per experiment). (B–C) ADCR assays were performed using PBMC effector cells and MDA-MB-231 target cells in the absence (B) or the presence (C) of IL-12. After 48-h supernatants were collected and IFNγ secretion was measured using ELISA. Data are representative of 2 independent experiments (duplicate measurements per experiment).