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. 2015 Apr 21;10(3):e990847. doi: 10.4161/15592324.2014.990847

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Figure 3. — Interaction of PVA31 and VAMP proteins. Yeast two-hybrid assay were performed to confirm between PVA31 and Arabidopsis VAMP proteins. PVA31 and VAMP/711/721/722/724/727 were fused with activation domain (AD) and DNA binding domain (BD) of the transcription factor GAL4, respectively. The yeast strains co-transformed with PVA31 and indicated VAMPs plasmids were grown on selective media lacking leucine, tryptophan and histidine and adding 3-AT. Positive control: pGADT7-T-antigen and pGBKT7-p53. Negative control: pGADT7-T-antigen and pGBKT7-Lamin C.