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Expression of marker genes for SA-mediated biotic stress responsive pathway are upregulated in PVA31-overexpressing plants with elicitor application A quantitative RT-PCR analysis of PR-1 gene was performed with total RNA isolated from a whole 14-day-old WT plant at various time points (0, 1, 6, 12, and 24 h) following flg22 treatment. UBQ10 was used as an internal control. Each vertical bars represented the mean ± standard error (3 leaves from each plant were used for the analysis).
