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. 2014 Dec 21;7(1):110–119. doi: 10.4161/19420862.2014.985919

Table 1.

Summary of binding kinetics of 50 mAbs

Hybridoma Id Antigen Ave. Kon(1/s) Ave. Koff(1/Ms) KD(M)
R28.1.1A10 HES1 415000 0.00371 3.83±0.29E-09
R159.1.4A11 HES1 4430 0.00149 9.75±7.02E-08
R247.3.1A3 HES1 30500 0.00157 5.82±2.05E-08
R16.1.2H10 STAT2 499000 0.00199 1.04±0.57E-08
R5.1.4C8 TGIF1 555000 0.00288 3.72±4.09E-09
R5.2.1B7 FOXO3 362000 0.0031 7.78±0.27E-09
R160.1.1C8 L3MBTL4 29000 0.00545 5.42±2.00E-08
R160.2.1C11 TSC22D4 378000 0.00389 1.48±0.70E-08
R16.1.2H10 STAT2 499000 0.00199 1.04±0.57E-08
R21.1.1E9 ARID3A 209000 0.00519 1.11±0.63E-08
R159.4.4B5 ZNF18 641000 0.00357 4.12±1.17E-09
R5.1.4C8 TGIF1 555000 0.00288 3.72±4.09E-09
R5.2.1B7 FOXO3 362000 0.0031 7.78±0.27E-09
R160.1.1C8 L3MBTL4 29000 0.00545 5.42±2.00E-08
R157.1.3D4 NRF1 39900 0.00157 5.17±1.40E-09
R157.1.3H3 NRF1 15100 1.4E-08 9.4±2.50E-12
R157.1.3H1 NRF1 33400 3.65E-09 <1.0E-13
R270.2.1D3 ZNF639 10400 1.09E-08 2.9±0.30E-12
R270.2.1E7 ZNF639 29600 0.000189 6.63±4.12E-09
R270.2.1F10 ZNF639 4070 0.000785 3.12±2.92E-08
R270.2.2B2 ZNF639 19600 1.48E-08 5.60±4.20E-13
R160.2.1C11 TSC22D4 21400 0.0011 1.48±0.70E-08
R154.1.3E10 TSC22D4 45600 0.000929 3.27±3.14E-08
R154.1.4A4 TSC22D4 43600 0.000339 4.62±3.51E-09
R147.1.2D9 COPS3 85300 8.2E-09 5.5±4.90E-13
R159.4.4B5 ZNF18 486000 0.00224 4.12±1.17E-09
R148.2.1E2 ZFYVE20 308000 0.00528 4.20±2.46E-08
R148.2.2E3 ZFYVE20 261000 0.00554 3.08±1.01 E-09
R148.2.1C3 ZFYVE20 127000 0.00356 3.49±0.45E-08
R148.2.2B9 ZFYVE20 871000 0.00268 1.98±0.51E-08
R159.4.1B8 PIKFYVE 31500 0.00296 1.02±0.76E-07
R147.1.2D9 COPS3 85300 8.2E-09 5.50±4.90E-13
R567.1.1D6 CXXC1 57400 1.09E-09 <1.0E-13
R567.1.1C7 CXXC1 72700 0.00637 9.58±5.64E-08
R567.1.1E5 CXXC1 382000 0.00139 6.96±5.01E-09
R567.1.1B6 CXXC1 62800 0.00511 8.19±5.05E-08
R567.1.2A7 CXXC1 118000 0.00387 3.27±3.03E-08
R567.1.2D3 CXXC1 47900 0.00208 7.27±6.38E-08
R567.1.1C5 CXXC1 444000 2.51E-08 3.93±1.30E-13
R567.1.2A1 CXXC1 25600 0.00237 3.54±3.00E-07
R451.1.1C10 BATF 85000000 0.00236 2.77±1.85E-07
R451.1.4G2 BATF 82300 2.74E-08 4.36±2.05E-13
R451.2.2E8 BATF 98000 0.00412 4.21±0.90E-08
R451.2.1E10 BATF 23500 1.21E-08 3.43±2.00E-13
R451.1.4B4 BATF 163000 2.42E-09 3.80±3.78E-15
R451.2.1H7 BATF 62600 0.00419 1.90±0.84E-07
536.2.1D9 CEBPE 2350 4.49E-08 1.90±1.00E-11
R359.1.1E4 DBX2 1500 0.00438 2.92±2.70E-06
R435.1.1A11 DVL3 135000 0.0028 1.54±1.03E-08
R329.1.4D10 BSX 411000 0.006 4.04±3.02E-07