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Epithelial cell expression of IL-8 after pneumococcal stimulation. IL-8 secretion from Detroit 562 human pharyngeal epithelial cells was stimulated by encapsulated strain D39 (serotype 2) as well as MNZ11 (PspK+) and MNZ1131 (PspK−). Epithelial cells were challenged with 1 × 107 pneumococci, and 4 h postchallenge, supernatants were collected and assayed by ELISA. Results are from two independent experiments done in triplicate and expressed as mean ± standard error (SE).
