FIG 5.

Antibody isotype response to rPspK immunization in mice. (A) Systemic immunization with rPspK led to PspK-specific antibody response. ELISA was used to determined PspK-specific serum IgG, IgM, and IgA levels. (B) Mucosal immunization with rPspK led to PspK-specific antibodies in mouse serum. (C) Mucosal immunization with rPspK led to PspK-specific antibody response in murine saliva. All samples were collected 3 weeks after the last immunization from a minimum of three mice. Anti-PspK antibodies were assayed in duplicate for each sample by ELISA. The results are expressed as the mean ± SE.