TABLE 1.

Proteins identified in the total extracts from stationary-phase promastigote and amastigote-like stages from Leishmania braziliensis

Protein namea	NCBI accession no.	Predicted mol mass (kDa)	Predicted pI	Parasite stageb		Leishmaniasis typec
				Promastigote	Amastigote-like	CL	ML
Enolase	XP_001563419.1	46.1	5.77	P	P	+	+
Actin	XP_001561807.1	21.0	5.11	P	P	+	+
Heat shock protein 83 kDa	XP_001567803.1	41.4	4.66	P	A	+	+
α-Tubulin	CAM45582	49.7	4.65	P	P	+	+
β-Tubulin	XP_001567862.1	49.8	4.45	P	P	+	+
Eukaryotic initiation factor 5a	XP_001565563.1	17.8	4.62	P	P	+	+
Calpain-like cysteine peptidase	XP_001563372.1	13,1	4.4	P	P	−	+
14-3-3 protein-like protein	XP_001568962.1	29.7	4.57	P	A	−	+
Iron superoxide dismutase	XP_001567555.1	21.7	6.62	P	A	−	+
Paraflagellar rod protein 1D	XP_001567017.1	69.3	5.21	A	P	+	+
Isocitrate dehydrogenase	XP_001568049.1	46.4	5.22	A	P	+	+
Cytochrome c oxidase subunit IV	XP_001563117.1	39.1	5.96	P	P	+	+
Hypothetical protein	XP_001566959.1	40.9	5.45	P	P	+	+
Translation elongation factor 1β	XP_001568778.1	23.0	4.32	A	P	+	+
Heat shock protein 70 kDa	XP_001566325.1	71.2	4.64	P	P	+	+
Putative heat shock protein 70	XP_001566324.1	27.0	4.89	P	P	+	+
Tryparedoxin peroxidase	XP_001563558.1	22,6	6.51	A	P	+	+
Hypothetical protein	XP_001563170.1	13.2	5.46	P	A	+	+
Proteasome activator protein pa26	XP_001568187.1	24.1	4.99	P	A	−	+
Receptor for activated C kinase 1	XP_001566321.1	34.4	6.37	P	A	−	+

^aName of the protein in L. braziliensis.

^bParasite stage in which the protein was identified. P, present; A, absent.

^cDiagnosed by the serum class used in the recognition of the protein. CL, cutaneous leishmaniasis; ML, mucosal leishmaniasis. + positive; −, negative.