Table 2.
Primer Sets Used
| Gene product | GenBank accession no. (NCBI) | Primer Sequences (5′→3′) | Length | Product size (bp) |
|---|---|---|---|---|
| GAPDH | NM_002046 | F: TCCTGCACCACCAACTGCTTAGC | 23 | 110 |
| R: GTCTTCTGGGTGGCAGTGATGG | 22 | |||
| α1C (CACNA1C) | NM_000719 | F: GGCTACCTGGATTGGATCACTC | 22 | 61 |
| R: TGCCTTCGTCCTCATTCTCAG | 21 | |||
| M3 (CHRM3) | NM_000740 | F: GTCACTCATTTCGGCAGCTACA | 20 | 51 |
| R: GGAGAAATTGCCAGCTGCTC | 20 | |||
| SM actin (ACTA2) | BC093052 | F: GCATCCTCATCCTCCCTTGAGAAG | 24 | 140 |
| R: GTTTCATGGATGCCAGCAGACTCC | 24 | |||
| SMMHC (MYH11) | AY520817 | F: CAGGAAACTTCGCAGTGATGCACC | 24 | 114 |
| R: CGTTTCCTCCTCAGAACCATCTGC | 24 |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; α1C, pore subunit of L-type Ca2+ channel; M3, muscarinic type 3 receptor; SM, smooth muscle; SMMHC, smooth muscle myosin heavy chain.