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. 2014 Oct 30;6(5):1201–1210. doi: 10.4161/mabs.29835

Figure 5.

Figure 5.

FcγRI specific cell killing. The HEK-293F cells (A) and the FcγRI-positive macrophage-like U937 cells (B) were cultured with gradient concentrations of PE38 (green), mFc-PE38 (blue) and mFc (red) at 37°C for 48 h. Cell viability assays were then performed to evaluate cell survival in response to the proteins. The mFc-PE38 fusion protein has potent cell killing to U937 cells but no killing effect to HEK-293F cells. (C) Schematic illustration of FcγRI specific cell killing mechanism. PE38 and mFc-PE38 cannot enter normal cells (left panel). The mFc-PE38 fusion protein binds to FcγRI, enters the FcγRI overexpressed cells by endocytosis, and is processed to release PE38 and causes cell death (right panel).