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Binding assay of HER2-specific Fabs isolated from the library. (A) ELISA screening of the five Fabs (m860, m863, m865, m868 and m869) isolated from the naïve library on coated HER2 ectodomain. (B) Competition ELISA showed m860 Fab could not be competed by Trastuzumab at 20μg/ml, while m860 IgG1 at the same concentration potently outcompeted the binding of m860 Fab to HER2.
