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6B8 Optimization design (a, light chain; b, heavy chain). CDRs as per the CCG definition are underlined. Amino acids different from the 6B8 hit are colored red if divergent or orange if similar. ∼: Sites where binary mutations (mouse and human) were included in the library. ≈: The site mutated to G, F, or Y to eliminate a potential deamidation motif, N27T28 (colored aqua).
