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Top selected optimized VH sequences. Mouse germ-line residues are shown in black, and human germ-line residues are in blue. Amino acids changed to remove a deamidation site are shown in green. VH sequences 02, 05, 36 and 65 were 95, 96, 96, and 98 percent identical to the human germ-line genes (IGHV1–69*08, HJ4), respectively, and had corresponding EpiVax scores of −17, −38, −17, and −34.
