Table 1.
Binding affinity and functional inhibition (IC50) of antibodies purified from representative hybridomas generated in the hybrid immunization campaign. Several antibodies with < 10 picomolar affinity and high potency to block IL23 induced IL17 production in mouse splenocytes assay were identified
| Hybridoma | Kd IL23 (pM) | Inhibition of IL23 induced IL17 production in mouse splenocytes (IC50, pM) |
|---|---|---|
| 18C4 | <10 | 56 |
| 18E5 | <10 | < 13 |
| 18D3 | <10 | < 13 |
| 20E8 | 640 | 499 |
| 22E2 | 1020 | 102 |
| 24A5 | 22 | 131 |
| 15C11 | 26 | 197 |
| 43F5 | 35 | 2000 |
| 27G8 | 27 | 235 |
| 31H9 | 12 | 960 |
| 9D12 | < 10 | 188 |
| 6B8 | < 10 | 8 |
| 26F7 | 28 | 9 |
| 34G3 | 33 | 8 |