Table 5.

Biophysical and Manufacturability Profile. Optimized antibodies could be expressed in NS0 cells with ease, had high monomeric content, solubilized to over 100 mg/mL concentrations in citrate based buffer, measure valance over +17.8 and thermal stability was in the range of 84–85°C

Assessment	BI 655066	BI 655067	BI 655068	BI 655069
Stable Pool Titer	300 mg/L	217 mg/L	227 mg/L	221 mg/L
AUC (% monomer)	98%	98%	96%	98%
Valence	+ 20.4	+ 22.5	+ 17.8	+ 19.6
Solubility (% monomer)	99% at 108 mg/ml	98% at 105 mg/ml	96% at 115 mg/ml	98% at 120 mg/ml
Thermal Stability	85°C	85°C	84°C	85°C