Figure 3.

Fruquintinib inhibited HUVEC tubule growth and CAM angiogenesis. Tube formation was suppressed significantly after treatment with fruquintinib at 0.3 μmol/L for 18 hours (A and B) No cytotoxicity was seen at the same concentration of fruquinitib in HUVECs. The plates were incubated for 3 hours at 37°C and fluorescence value was read at Ex 530 nm and Em 590 nm on Tecan (C) Fruquintinib displayed strong inhibition on the development of new blood vessels in the chick embryo (D) Left and right panels, as arrows indicated, were treated with saline and compound, respectively. Pseudolarix acid B was used as a positive control.