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Immunofluorescence and cell viability of human dermal fibroblasts. (A) Human dermal fibroblasts expressing the M2 muscarinic receptor. Blue, DAPI; Red, M2 receptor. (B) Human dermal fibroblast cell viability/proliferation in presence of different concentrations of Arecaidine. Gallamine (10−6M) was used as M2 antagonist to counteract the Arecaidine effects. Ctl, control, Are, Arecaidine, Gal, Gallamine, O.D., optical density.
