Figure 2.

Comparison of NAD(P)(H) pool and the disease development between PBI-type and SeNHX1 transgenic tobaccos. (A) Symptoms on tobacco leaves of PBI-type and 3 SeNHX1 transgenic lines (TS1, TS11, and TS15) at 0 and 60 hpi. Left side of leaf with wounding-only treatment was served as control (Scale bar = 2 cm). (B) Area of wilt spots after Ppn inoculation. (C) Evaluation on disease courses. The rank of disease symptom in leaves was classified into 3 levels from Rank 1 to 3 according to area of wilt spots.⁹ The higher rank means more serious infection. (D) H₂O₂ contents in leaf tissue at 0 and 60 hpi. Data are means ± SE (n = 30 leaves). (E) The content of NAD(P)(H) components. (F) NAD(P)(H) pool as NAD(P) + NAD(P)H. Data are means ± SE (n = 6 seedlings). PBI means tobacco plants transformed with pBI121 empty vector. TS1, TS11, and TS15 represent T1 progenies of SeNHX1 transgenic tobacco lines, respectively. Asterisks on bars indicate significant differences from PBI-type plants under the same treatment at P ≤ 0.05.