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PDADMAC flocculent settling profile for cell broth diluted with different diluents. Cell broth was diluted to 33% with cell culture media (CCM) [♦], 9% (w/v) sucrose [▪], 32 g/L betaine [▴], 10% (w/v) dextran 70 with 30 g/L betaine [•], and 14.6% (w/v) PEG 1,000 [X]. Diluents' stock concentration was based on an osmolality of 300 mOsmo/kg. The lactate concentration at the end of cell culture production was 9 g/L.
