Table 4.

PDADMAC level's measured in process intermediates from a typical mAb purification process. Flocculation harvest PDADMAC concentration was 45 pg/TC and PEG 3,000 concentration was 3% (w/v). PDADMAC level are in ppb. ND is not determined

	PDADMAC-only		PEG / PDADMAC
Unit Operation	Depth Filtered	Non-Depth Filtered	Non-Depth Filtered
Harvest Pool	5,000 ± 30%	ND	ND
Protein A Pool	500 ± 30%	5,000 − 10,000 ± 30%	≤50 ± 30%
n-aPA	100 ± 30%	ND	ND
Depth Filtered n-aPA	≤50 ± 30%	ND	ND
DS	≤120 ± 30%	ND	ND