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mAb Protein A pool product quality comparison of Protein A load prepared by centrifugation followed by a two-staged filtration train or PDADMAC flocculation followed by membrane filtration. Flocculation harvest PDADMAC-only concentration was 45 pg/TC
| SEC |
CEX-HPLC |
SDS-CE |
||||||
|---|---|---|---|---|---|---|---|---|
| Harvest Method | HMW | LMW | HCP (ppm) | Host DNA (ppm) | Acidic | Basic | Non-Reduced | Reduced |
| Centrifugation | 11.5% | 1.6% | 5,703 | 75,000 | 9.8% | 10.8% | 5.8% | 5.8% |
| Flocculation | 11.6% | 1.4% | 6,164 | 140 | 9.4% | 10.1% | 5.9% | 5.7% |
