Table 6.

Modeling the effect of cell broth solid levels on the harvest volume for a centrifugation, MF and flocculation harvest process. All harvest volumes are relative to the bioreactor volume. Minimum harvest recovery used in this modeling is ≥90%. For centrifugation harvesting, a cell broth dilution is required to enable processing of the feed at 10–12% solids. Modeling the MF harvest volumes was based on 100% mAb sieving. PDADMAC flocculation harvest volumes based on the entrapped supernatant (VE) using the equations described in the Methods section. PDADMAC flocculation required a minimum dilution of 10% for the addition of PDADMAC

	Centrifugation		MF		PDADMAC Flocculation
Bioreactor Solids	10% Solids	12% Solids	3 DF	4 DF	38% VE	19% VE
40%	3.60	2.93	1.80	2.40	1.45	0.86
20%	1.80	1.47	2.40	3.20	0.84	0.87
10%	0.90	0.90	2.70	3.60	0.97	0.99