Table 1.
Characteristics of 7 model human IgG mAb clones evaluated in this study. For the fourth and fifth columns, the identity (in percentage) to the corresponding germline sequence is shown on a gene-segment-by-gene-segment basis
| mAb | Antigen class | HC sublcass / LC isotype | V-D-J gene usage for VH (identity to germline) | V-J gene usage for VL (identity to germline) | Calculated isoelectric point | Identified unfavorable solution properties |
|---|---|---|---|---|---|---|
| A | cytokine receptor | G2 / kappa | VH4|4-59/D3|3-22|RF2/JH2 (91.8%/33.3%/93.8%) | VK3|A27/JK1 (89.4%/92.3%) | pH 8.93 | low solubility at neutral pH |
| B | integrin | G2 / kappa | VH3|3-11/D6|6-19|RF1/JH4 (96.9%/80.0%/100%) | VK1|L19/JK1 (94.6%/100%) | pH 8.39 | low solubility at neutral pH |
| C | cytokine receptor | G2 / kappa | VH1|1-24/D3|3-3|RF2/JH4 (92.9%/33.3%/86.7%) | VK2|A2/JK4 (96.9%/100%) | pH 6.58 | agitation sensitivity |
| D | growth factor receptor | G2 / kappa | VH1|1-18/D3|3-3|RF3/JH4 (100%/40%/93.3%) | VK4|B3/JK3 (97.0%/100%) | pH 7.0 | agitation sensitivity |
| E | cytokine receptor | G2 / kappa | VH1|1-24/D6|6-6|RF1/JH4 (96.9%/70%/93.3%) | VK4|B3/JK1 (93.9%/100%) | pH 6.33 | high viscosity |
| F | secreted factor | G2 / kappa | VH1|1-18/D7|7-27|RF1/JH6 (83.7%/33.3%/73.7%) | VK1|O2/JK4 (90.3%/92.3%) | pH 7.05 | crystallization at neutral pH |
| G | secreted factor | G1 / kappa | VH3|3-48/D4|4-17|RF2/JH4 (92.9%/60%/80%) | VK2|A19/JK2 (99.0%/92.3%) | pH 8.51 | no notable issues |