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. 2015 May 18;10(7):622–632. doi: 10.1080/15592294.2015.1050173

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Figure 6. — Promoter methylation and mRNA expression of NEFH, NEFL and NEFM in various types of cancer. (A) Promoter methylation and mRNA expression of NEFH, NEFL and NEFM was assayed in 3 representative cell lines of each cancer type by MSP and RT-PCR, respectively. U and M marking unmethylated and methylated bands, respectively. Basal expression of NEFH, NEFL, and NEFM was confirmed in normal tissue of each cancer type. GAPDH expression was assayed as control. (B) Overall DNA methylation frequency (in %) of NEFH, NEFL, and NEFM in pancreas, gastric and colon cancer cell lines. (C) Overall DNA methylation frequency (in %) of NEFH, NEFL and NEFM in primary tissue of pancreas (n = 30), gastric (n = 30), and colon cancer (n = 128).