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. 2015 Feb 18;16(3):392–401. doi: 10.1080/15384047.2014.1002358

Figure 5.

Figure 5.

USP9X inhibition impacts apoptotic cell death in NSCLC cells expressing high but not low MCL1. (A) Western blot showing effect of USP9X siRNA knockdown on MCL1 expression in cell lines arranged by basal MCL1 expression. (B) Luminescent cell viability assays in a low MCL1 expressing line, NCI-H322 (left), compared to a high MCL1 expressing line, NCI-H1299 (right), treated with IR with or without USP9X siRNA knockdown. Error bars represent standard deviation. (C) Apoptosis in a low MCL1 expressing line, NCI-H322 (left), compared to a high MCL1 expressing line, NCI-H1299 (right), measured as a percentage of cells staining positive on flow cytometry for annexin V-FITC following fractionated IR over 3 consecutive days with or without USP9X siRNA knockdown (top) or WP1130 treatment (bottom). Error bars represent standard deviation. (D) Caspase 3/7 activity in NCI-H322 (left) and NCI-H1299 (right) cells measured by Caspase-Glo 3/7 luminescent assay (Promega) following single fraction IR with or without USP9X siRNA knockdown (top) or WP1130 treatment (bottom). Error bars represent standard deviation.