Figure 1 (See previous page).

Oxaliplatin induces the autophagy in colorectal cancer cells and inhibition of autophagy enhances the chemotherapy sensitivity. (A) HT29 and HCT116 were treated with the indicated concentration of oxaliplatin for 24 hours. At the end of treatment, cell lysates were prepared, resolved by SDS-PAGE, and subjected to Western blot analysis using anti-LC3, anti-p62 and anti-actin antibodies, respectively. Actin was used as a loading control. (B) HT29 and HCT116 cells were treated with the indicated concentrations of oxaliplatin for 24 h in the presence or absence of 3-MA (2 mM).At the end of treatment, cell viability was measured by CCK8. (C) HT29 and HCT116 were treated with oxaliplatin (35 µM) for 24 hours in the presence or absence of 3-MA (2 mM) and then the level of apoptosis was determined by flow cytometric analysis of Annexin V staining.