The mRNA expression levels of the 5 radioresistance-related genes in Hep-2 and RR-Hep-2 cells were positively associated with 5-Aza treatment. Hep-2 and RR-Hep-2 cells were treated with or without 5-Aza (5 μM) for 72 h, and (A) RT-PCR analysis was used to assess the mRNA expression levels of TOPO2A, PLXDC2, ETNK2, GFI1, and IL12B. (B) The mRNA levels of TOPO2A, PLXDC2, ETNK2, GFI1, and IL12B were measured by quantitative real-time PCR.