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. 2015 Feb 26;16(4):558–566. doi: 10.1080/15384047.2015.1017154

Table 1.

Gene pyrosequencing conditions and primers

Gene name Accession number Start (genomic) Strand Sequence (5'~3') Tm (°C) product (bp)
ETNK2 NM_018208.1 1819169 Sense TTTATGGGGTATGGAGGAGAA 58 226
1819394 Anti-Sense CCTAACAAAACCCCATCCT  
GFI1 NM_005263.2 62924153 Sense TGTTGTGGAAATATTTGGGGATAA 53 121
62924273 Anti-Sense CCACCTCTTCCAAATTTAACCAT    
IL12B NM_002187.2 3567357 Sense TAG ATT TTT TGG GTT TTA TA 55 168
3567524 Anti-Sense TAC TAA ACC CTT TAC CCT TC    
PLXDC2 NM_032812.7 19790463 Sense TAG GAA ATT TAG TGG GTT TTT 57 193
19790655 Anti-Sense AAT AAA AAA AAC AAA CCA AAA CC    
TOPO2A NM_001067.2 1184943 Sense AGGTTGGAGTGTAGTGGAGTAA 59 185
1185128 Anti-Sense TTAAAAACCAACCTAAACAACATAA