Table 2.
PCR conditions and primers
| Gene name | Sequence (5'->3') | Tm (°C) | Product size (bp) | |
|---|---|---|---|---|
| ETNK2 (F) | ATACTATCCACGCCAACGGC | 58 | 556 | |
| ETNK2 (R) | CGGATCACTGCGTACCTGAG | |||
| GFI1 (F) | GAACGGAGCTCGGAGTTTGA | 54 | 988 | 1st |
| GFI1 (R) | CCACCTTCCTCTGGAAACCC | |||
| GFI1 (F) | TCGGAGAAGTCAATGTGCCC | 53 | 847 | 2nd |
| GFI1 (R) | GGTTGGAGCTCTGGCTGAAT | |||
| IL12B (F) | GTCACAAAGGAGGCGAGGTT | 58 | 497 | 1st |
| IL12B (R) | GCTGACCTCCACCTGCCGAG | |||
| IL12B (F) | GTCACAAAGGAGGCGAGGTT | 58 | 160 | 2nd |
| IL12B (R) | CAGCAGGTGAAACGTCCAGA | |||
| PLXDC2 (F) | TGTGGCAACCGGGGGTTTCA | 61 | 578 | |
| PLXDC2 (R) | TGTCCACCCAGTCCTGCCGAT | |||
| TOPO2A (F) | ACAGAGCCACGGAGAGCAGCA | 58 | 590 | |
| TOPO2A (R) | TTTGCCCGAGGAGCCACAGC |