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. 2015 Jan 20;10(1):62–71. doi: 10.4161/15592294.2014.990780

Figure 6.

Figure 6.

PCAF deficiency inhibits the palmitate-induced recruitment of H3K18ac on the promoters of ICAM-1 and MCP-1 and upregulations of these inflammatory molecules. (A) Quantitative results for the H3K18ac ChIP assay in shScram and shPCAF cells. (B) qPCR analysis for ICAM-1 and MCP-1 in HK-2 cells treated with 300 μM PA. Quantitative results of the H3K18ac ChIP assay on the promoters of ICAM-1 (C) and MCP-1 (D) in shScram and shPCAF cells treated with 300 μM palmitate. (E) Representative western blots for PCAF, VCAM-1, ICAM-1, NF-κB p50, MCP-1, p-p38, and β-actin in the shScram and shPCAF cells treated with 300 μM palmitate (left panel), with their densitometric quantitative results (right panel). (shScram, cells stably transfected with pSUPER-shScram; shPCAF, cells stably transfected with pSUPER-shPCAF; Mock, cells treated with vehicle; PA, palmitate treatment. Each experiment was performed in duplicates for 3 times with a representative result shown. * P < 0.05 compared with Mock group. # P < 0.05 compared with PA-treated cells)