Figure 1.

Undetectable Histone O-GlcNAcylation following OGT and TET2 overexpression. (A) HEK293T cells were transfected with Flag-H2B along with pcDNA3 empty vector, Myc-OGT or Myc-OGT catalytic dead (CD), as well as Myc-TET2 alone or in combination with Myc-OGT. Three days posttransfection, cells pellets were harvested and immunoprecipitation (Flag-IP) following protein denaturation was conducted to obtain purified Flag-H2B. The immunopurified histones were subjected to western blotting analysis using the indicated antibodies. Dots indicate Myc-OGT and endogenous OGT (eOGT).