Figure 2.

Competition ELISA experiments. (A) Results from an analysis of the ability of selected affibody variants, or the mAb tocilizumab, to compete with the interaction between IL-6 (biotinylated) and the IL-6Rα (B) Results from an analysis of the ability of selected affibody variants, or the mAb tocilizumab, to compete with the interaction between preformed IL-6 (biotinylated)/sIL-6Rα complexes and gp130 (gp130-Fc). For each construct, four concentrations were used: 500 nM (black bars), 50 nM, 5 nM and 0.5 nM (lightest gray bars).