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. 2014 Nov 3;6(6):1474–1485. doi: 10.4161/mabs.36313

Figure 4.

Figure 4.

Comparison of LC separation and quantification of 2-AB labeled free glycans released from the control and biosimilar CTLA4-Fc fusion proteins. (A) The fluorescence chromatograms of 2-AB labeled glycans separated by the UPLC HILIC column from intact protein. (B) Integrated peak area of LC-fluorescence chromatograms, relative contents of 2-AB labeled glycans were quantified (n = 3). (C) The fluorescence chromatograms of 2-AB labeled glycans separated by the UPLC HILIC column from the 2 separation part.