Table 1.

Purification yields of modeled mAbs and cytotransmabs that were obtained by transient expression in HEK293F cells

IgG clones	VH of HCa	VL of LCa	IgG purification yield (mg/1 L of transfected cells)b,c
TMab3	h3D8	hT3	8.2 ± 0.5
TMab4	h3D8	hT4	10.8 ± 1.0
Adalimumab	Adalimumab	Adalimumab	11.6 ± 0.3
HuT3	Adalimumab	hT3	3.5 ± 0.8
HuT4	Adalimumab	hT4	10.9 ± 0.8
Bevacizumab	Bevacizumab	Bevacizumab	8.8 ± 0.4
AvaT3	Bevacizumab	hT3	4.5 ± 0.8
AvaT4	Bevacizumab	hT4	8.0 ± 1.1
C20	C20	C20	22.5 ± 2.5
KT4	C20	hT4	21.3 ± 0.9

^aThe constant domains of HC and LC were the IgG1-isotype and Cκ, respectively.

^bThe 2 plasmids that encode the HC and LC of each IgG antibody were co-transfected with the equivalent molar ratio into HEK293F cells in 1 L of culture media following standard protocol.⁵⁰

^cAfter 7 d of culture, antibodies were purified from the cell culture supernatant using a protein-A affinity column. The values represent mean ± SD of at least 3 independent experiments.