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. 2015 Jan 29;10(1):92–101. doi: 10.1080/15592294.2014.998531

Figure 3.

Figure 3.

Phenotype analysis of Hoxb7a-overexpression in zebrafish embryos. (A) Phenotype analysis at 72 hpf of Hoxb7a mRNA injected zebrafish resulted in significant hypopigmentation and malformation in 66% of the injected zebrafish. These embryos had shorter anterior/posterior axes as well as crooked or bent tails. (B) Phenotype analysis after pax2a staining at 24 hpf resulted in about 63% embryos with a mild or severe affected phenotype after Hoxb7a overexpression compared to 13% in injected controls. (C) Pax2a staining after microinjection of different concentrations of mRNA. From left to right severe, mild affected and wild type (WT) embryos at 24 hpf. WT zebrafish show expression in the hindbrain, hindbrain-midbrain boundary, neural tube, mesoderm, optic stalk, otic vesicle, and pronephric duct. Microinjection 62.5 μM mRNA, 125 μM mRNA and 250 μM mRNA resulted in respectively 48%, 71% and 61% malformed zebrafish. There was no correlation between mRNA dosage and severity of malformation.