Table 1.
Background information of MMC patients included in the HumanMethylation450 BeadChip and Sequenom EpiTYPER
| MMC patient |
Sibling |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MMC patient | Type MMC | Hy/VP | ACM | Scoliosis | ADL | UI | Ethnicity | Maternal age (years) at birth of MMC patient | MTHFR 677C>T | Gender | MTHFR 677C>T | Gender | MMC patient versus sibling age (months) |
| 1*+ | S | yes | 1 | yes | 2 | yes | Belgian | 36 | CC | F | |||
| 2*+ | LS | yes | 2 | yes | 3 | yes | Belgian | 29 | CT | F | |||
| 3*+ | LS | yes | 2 | yes | 3 | yes | Moroccan | 32 | CT | M | |||
| 4*+ | LS | yes | 1 | yes | 3 | yes | Indonesian | 26 | CC | F | |||
| 5*+ | LS | yes | 2 | no | 2 | yes | Belgian | CC | M | CC | F | −18 | |
| 6*+ | LS | yes | 2 | yes | 3 | yes | Belgian | 27 | CC | F | |||
| 7*+ | LS | yes | 1 | yes | 3 | yes | Belgian | CC | M | ||||
| 8*+ | S | yes | 2 | no | 1 | yes | Belgian | 27 | CC | F | |||
| 9* | S | no | 0 | no | 1 | yes | Belgian | TT | M | ||||
| 10* | LS | yes | 2 | no | 2 | yes | Belgian | 25 | CC | M | |||
| 11+ | LS | yes | 2 | yes | 3 | yes | Belgian | 27 | CC | M | |||
| 12+ | LS | yes | 2 | yes | 3 | yes | Turkish | 28 | CC | F | |||
| 13+ | S | yes | 0 | no | 1 | yes | Belgian | 33 | CT | M | |||
| 14+ | LS | yes | 2 | yes | 3 | yes | Belgian | CC | F | ||||
| 15+ | LS | yes | 2 | yes | 3 | yes | Turkish | 20 | CT | F | |||
| 16+ | S | yes | 2 | yes | 3 | yes | Belgian | TT | M | ||||
| 17+ | LS | yes | 2 | yes | 3 | yes | Belgian | 25 | CT | F | |||
| 18+ | S | yes | 2 | yes | 2 | yes | Belgian | 28 | CC | M | |||
| 19+ | LS | yes | 2 | no | 1 | yes | Belgian | 36 | CT | M | |||
| 20+ | LS | yes | 2 | yes | 1 | yes | Belgian | CT | M | ||||
| 21+ | LS | yes | 2 | yes | 3 | yes | Belgian | 36 | CT | F | |||
| 22+ | TL & LS | yes | 2 | yes | 3 | yes | Belgian | 23 | CC | M | |||
| 23+ | S | no | 0 | yes | 1 | yes | Belgian | 30 | CT | M | |||
| 24+ | LS | yes | 2 | yes | 2 | yes | Belgian | 25 | CT | F | |||
| 25+ | LS | yes | 2 | no | 2 | yes | Belgian | 34 | CC | F | |||
| 26+ | LS | no | 0 | no | 1 | yes | Belgian | CC | F | ||||
| 27+ | LS | yes | 2 | yes | 3 | yes | Belgian | CT | M | CC | F | −20 | |
| 28+ | LS | yes | 2 | yes | 3 | yes | Moroccan | CT | F | ||||
| 29+ | LS | yes | 2 | yes | 3 | yes | Belgian | 31 | CC | M | CC | M | −19 |
| 30+ | LS | yes | 0 | no | 1 | yes | Mongolian | CC | F | ||||
| 31+ | LS | no | 0 | no | 1 | no | Belgian | 20 | TT | M | CC | M | −20 |
| 32+ | LS | yes | 2 | no | 1 | yes | Belgian | 22 | CC | M | |||
| 33+ | LS | yes | 2 | yes | 2 | yes | Belgian | 28 | CT | M | |||
| 34+ | LS | yes | 2 | yes | 3 | yes | Belgian | CT | F | ||||
| 35+ | S | yes | 2 | no | 1 | yes | Belgian | 33 | CT | M | |||
| 36+ | LS | yes | 2 | no | 3 | yes | Belgian | 27 | CC | M | |||
| 37+ | LS | yes | NA | yes | 2 | yes | Belgian | 26 | CT | F | |||
| 38+ | LS | yes | 2 | yes | 2 | yes | Belgian | CT | F | ||||
| 39+ | LS | yes | 2 | yes | 3 | yes | Belgian | 28 | CT | F | |||
| 40+ | LS | yes | 2 | no | 1 | yes | Belgian | 32 | CC | M | |||
| 41+ | LS | no | NA | no | 3 | yes | Belgian | 36 | CC | M | CT | M | −26 |
| 42+ | L | yes | 2 | yes | 3 | yes | Ukrainian | 20 | CC | F | |||
| 43+ | LS | yes | 2 | no | 1 | yes | Belgian | 25 | CC | F | TT | M | 20 |
| 44+ | LS | yes | 2 | yes | 3 | yes | Belgian | CC | M | ||||
| 45+ | LS | yes | 2 | yes | 3 | yes | Belgian | 29 | CT | F | |||
| 46+ | LS | yes | 2 | yes | 3 | yes | Belgian | CC | F | ||||
| 47+ | S | yes | 2 | no | 1 | yes | Moroccan | 31 | CT | F | |||
| 48+ | LS | yes | 2 | yes | 2 | yes | Belgian | 27 | CT | F | |||
| 49+ | S | yes | 2 | yes | 3 | yes | Belgian | 34 | CC | F | |||
| 50+ | S | no | NA | no | 1 | yes | Belgian | 36 | CT | F | CC | F | 17 |
| 51+ | LS | yes | 2 | no | 3 | yes | Belgian | 30 | CC | F | |||
| 52+ | LS | yes | 2 | no | 1 | yes | Moroccan | 24 | CC | F | |||
| 53+ | S | yes | 2 | no | 1 | yes | Belgian | 28 | CC | F | |||
| 54+ | S | no | NA | no | 1 | yes | Belgian | CT | F | ||||
| 55+ | LS | yes | 2 | no | 2 | yes | Bosnian | 31 | CT | F | CC | M | 53 |
| 56+ | S | no | 0 | no | 1 | yes | Belgian | 30 | CC | F | |||
| 57+ | LS | yes | 2 | no | 1 | yes | Belgian | 24 | CC | F | |||
| 58+ | S | no | 2 | no | yes | Moroccan | 26 | CC | F | ||||
| 59+ | LS | yes | 2 | yes | 3 | yes | Belgian | 27 | CC | F | |||
| 60+ | LS | yes | 2 | no | 1 | yes | Turkish | 35 | CT | F | CC | F | −101 |
| 61+ | LS | yes | 2 | yes | 3 | yes | Belgian | CT | F | ||||
| 62+ | LS | yes | 2 | no | 1 | yes | Turkish | 29 | CT | F | |||
| 63+ | LS | yes | 2 | yes | 3 | yes | Belgian | 35 | CT | F | |||
| 64+ | L & CP | yes | 2 | no | 2 | yes | Belgian | 40 | CT | F | CT | F | −27 |
| 65+ | LS | yes | 2 | yes | 2 | yes | Belgian | CT | F | ||||
| 66+ | LS | yes | 2 | yes | 2 | yes | Belgian | 24 | CT | F | |||
| 67+ | LS | yes | 2 | yes | 2 | yes | Belgian | CT | F | ||||
| 68+ | S | yes | 2 | no | 1 | no | Belgian | CT | M | ||||
| 69+ | LS | yes | 2 | yes | 3 | yes | Belgian | 24 | CT | M | |||
| 70+ | LS | yes | 2 | yes | 3 | yes | Belgian | 29 | CC | M | |||
| 71+ | LS | yes | 2 | yes | 3 | yes | Belgian | 33 | CC | F | |||
| 72+ | LS | yes | 2 | yes | 2 | yes | Belgian | 29 | CC | M | |||
| 73+ | LS | yes | 2 | yes | 3 | yes | Belgian | 29 | CT | F | CT | F | −24 |
| 74+ | LS | yes | 2 | yes | 2 | yes | Belgian | CT | M | ||||
| 75+ | LS | yes | 2 | no | 1 | yes | Belgian | CC | F | ||||
| 76+ | LS | yes | 2 | yes | 2 | yes | Belgian | 31 | CT | F | |||
| 77+ | LS | yes | 2 | yes | 3 | yes | Belgian | 26 | CC | M | |||
| 78+ | LS | yes | 2 | yes | 3 | yes | Belgian | 30 | CT | M | |||
| 79+ | S | no | 0 | no | 1 | yes | Belgian | CT | M | ||||
| 80+ | LS | yes | 2 | no | 1 | yes | Belgian | 30 | CC | F | |||
| 81+ | LS | yes | NA | no | 2 | yes | Turkish | CT | M | ||||
| 82+ | TL | yes | 2 | yes | 3 | yes | Belgian | TT | F | ||||
| 83+ | TL | yes | 2 | yes | 1 | yes | Belgian | 24 | TT | F | |||
| 84+ | TL | yes | 1 | yes | 3 | yes | Belgian | 37 | CC | F | |||
| 85+ | TL | yes | 2 | no | 1 | no | Belgian | TT | F | ||||
*
Inclusion in HumanMethylation 450K BeadChip (10 MMC patients) and +Sequenom EpiTYPER (83 MMC patients); MMC: myelomeningocele; M: male; F: female; S: Sacral; LS: Lumbosacral; TL: Thoracolumbal; CP: Cheilopalatoschisis; Hy/VP: presence of hydrocephaly and ventriculoperitoneal drain; ACM: Arnold Chiari Malformation: 1 = type 1, 2 = type 2; NA: Not Available; ADL: Activities of daily life: 1 = ambulatory, 2 = household ambulatory with wheelchair for longer distances, 3 = wheelchair dependent; UI: urinary incontinence; MTHFR 677C>T genotype (CC/CT/TT) in 85 MMC patients: 47% CC - 53% CT/TT vs. 40% CC - 60% CT/TT in 30 healthy unrelated controls (P-value = ns).