Table 1.

Fc glycan composition of the wild-type TZM and F243A mutant expressed alone or co-expressed with GT, ST6 or both. The values are averages of relative peak heights in the molecular weight profiles obtained by LC-ESI-MS of Fc/2 glycopeptides of 2 independent batches. The category “other tri-antennae” contains glycans having a third complex branch being partially sialylated or galactosylated (e.g. G3FS2). High-mannose glycans comprise M5, M6, M7, M8 and M9 The category “truncated core” contains the hybrid glycans Galβ1–4GlcNAcβ1–2Manα1–3/6(Manα1–6/3)Manβ1–4GlcNAcβ1–4(Fucα1–6)GlcNAc (abbreviated here as G1F−GlcNAc), GlcNAcβ1–2Manα1–3/6(Manα1–6/3)Manβ1–4GlcNAcβ1–4(Fucα1–6)GlcNAc (G0F−GlcNAc), and GlcNAcβ1–2Manα1–3/6Manβ1–4GlcNAcβ1–4(Fucα1–6)GlcNAc, as well as the Fucα1–6GlcNAc fragment (GlcNAcF), which was found in one of the 2 batches only, but represented 2 to 6% in the wild-type mab, and up to 10–15% in the mutant

	TZM (%)	TZMGT (%)	TZMST6 (%)	TZMGTST6 (%)	TZMm (%)	TZMmGT (%)	TZMmST6 (%)	TZMmGTST6 (%)
G0F	54.4 ± 1.9	15.0 ± 1.4	49.6 ± 4.6	12.3 ± 1.4	8.3 ± 2.3	0.0	9.9 ± 0.2	0.6 ± 0.8
G1F	31.4 ± 2.1	23.5 ± 4.2	26.3 ± 1.5	19.5 ± 1.4	19.4 ± 0.4	3.5 ± 0.3	14.9 ± 2.9	1.4 ± 0.3
G2F	5.1 ± 0.1	44.8 ± 2.1	3.5 ± 0.1	20.9 ± 2.9	11.4 ± 1.1	41.0 ± 0.5	2.3 ± 0.1	2.9 ± 0.4
G3F	0.0	0.0	0.0	0.0	0.0	2.2 ± 0.0	0.0	0.0
G1FS1	0.9 ± 1.3	0.6 ± 0.8	5.8 ± 0.7	2.4 ± 0.3	14.3 ± 4.3	2.2 ± 0.4	19.8 ± 3.3	2.8 ± 0.6
G2FS1	0.0	3.5 ± 1.2	2.7 ± 0.7	32.0 ± 1.0	16.7 ± 0.6	19.9 ± 5.6	13.4 ± 1.3	29.1 ± 4.0
G2FS2	0.0	0.5 ± 0.7	0.0	6.6 ± 0.8	19.6 ± 0.2	17.0 ± 6.3	28.6 ± 1.2	50.4 ± 4.4
G3FS3	0.0	0.0	0.0	0.0	4.3 ± 0.8	1.3 ± 1.8	5.0 ± 0.7	2.6 ± 1.4
Other tri-antennae	0.0	0.0	0.0	0.0	0.0	0.0	1.1 ± 0.1	2.6 ± 1.0
High-mannose	5.3 ± 1.7	6.8 ± 4.2	4.8 ± 1.3	4.7 ± 2.7	0.6 ± 0.8	4.4 ± 2.7	0.0	2.0 ± 2.9
Truncated core	2.9 ± 1.0	5.3 ± 4.7	6.9 ± 3.6	1.6 ± 2.2	5.4 ± 7.6	8.6 ± 12.2	5.0 ± 7.1	5.7 ± 8.0