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. 2014 Dec 18;7(1):120–128. doi: 10.4161/19420862.2015.989028

Figure 1.

Figure 1.

Effect of IgG subclass on FVIII-mimetic activity. Effect of each antibody on FIXa-catalyzed FX activation in the presence of FIX, FIXa, FX, and synthetic phospholipid is shown. The X-axes indicate the concentration of antibody in the FX activation reaction. The Y-axes indicate the absorbance at 405 nm after the addition of chromogenic substrate S-2222, which represents the generation of FXa. (A) The parent human IgG4 antibody with Ser228Pro (black circles) shows the antibody concentration–dependent FXa generation, described as the FVIII-mimetic activity. The FVIII-mimetic activity of the human IgG1 variant (red squares), and the human IgG2 variant with the IgG1-like disulfide bond pattern (blue diamonds) is shown. (B) The FVIII-mimetic activity of the parent IgG4 antibody (black circles) and the different subclass combinations: IgG1/2 (green triangles), IgG1/4 (purple circles), IgG2/1 (yellow squares), IgG2/4 (pink diamonds), IgG4/1 (light blue triangles), and IgG4/2 (light green circles) is shown. The number before the slash indicates the subclass of the Q chain, and the number after the slash indicates the subclass of the J chain, e.g. the Q and J chain subclasses of IgG1/2 are IgG1 and IgG2, respectively.