Table 1A.

scFvs isolated from panning naïve antibody phage display libraries

	2 panning rounds (peptide/peptide)		3 panning rounds (peptide/peptide/cell)
	no. of unique1 scFv's binding to		no. of unique1 scFv's binding to
antigen	antigen	hCXCR22	antigen	hCXCR22
A	32	9 (28%)3	31	17 (55%)
B	41	3 ( 7%)	28	23 (82%)

¹Uniqueness initially assessed by fingerprinting, subsequently confirmed by sequencing. ²Binding to HEK293 cells stably expressing huCXCR2, but not to the parental cell line.³Percentage subpopulation antigen binding clones cross-reacting with CXCR2 overexpressing cells, but not the parental cells.