Figure 5.

Plot of the total fragment data for mAb 2 and mAb 2 H224S, as determined by SEC. Samples were incubated in 20 mM sodium acetate, pH 5.3, in the absence or presence of 0.04 mM Cu²⁺ and stored at 40°C. Aliquots were examined at various time points for up to 2 weeks. The R² values for the mAb 2 wt and mAb 2 H224S incubated with Cu²⁺ are 0.97 and 0.84 and without Cu²⁺ are 0.78 and 0.80, respectively.