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. 2015 Apr 22;16(6):987–996. doi: 10.1080/15384047.2015.1030552

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Figure 3. — GATA-3 negatively regulates Cx43 by directly binding to its promoters. (A, B) Quantitative RT-PCR analysis of GATA-3 (A) and Cx43 (B) levels in H. pylori infected GES-1 cells treated with a scramble control, GATA-3 siRNA-1, GATA-3 siRNA-2. (C) Western blot analysis of GATA-3 and Cx43 protein levels in H. pylori infected GES-1 cells treated with a scramble control, GATA-3 siRNA-1, and GATA-3 siRNA-2. (D) GATA-3 direct binds to Cx43 promoters. HEK-293T cells co-transfected with GATA-3 and Cx43-pGL3 plasmids. The results are displayed as the ratio of firefly luciferase activity in the pGL3-Cx43-GATA-3-transfected cells to the activity in the pGL3-Cx43 controls. *** p < 0.001.