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. 2015 May 5;7(5):957–968. doi: 10.1080/19420862.2015.1045168

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Figure 4. — Anti-KDR antibody, TTAC-0001, inhibits VEGF-induced phosphorylation of VEGFR-2 and downstream signaling molecule in HUVECs. HUVECs were pretreated for 30 min with various concentrations of TTAC-0001 anti-KDR neutralizing antibody and stimulated with 10 ng/ml of VEGF for 10 min. (a) Anti-KDR/Flk-1 immunoprecipitates (IP) were analyzed by SDS-PAGE. Immunoblot analysis was performed with anti-phosphotyrosine antibody (anti-PY20). (b) Activation of ERK kinase by VEGF was determined by Western blotting. Various amounts of antibody were pre-treated and stimulated with VEGF.